Bioline Reagents is a primary manufacturer of specialised molecular biology products for the life science industry and research markets. It manufactures reagents including ultrapure nucleotides, DNA polymerases and mixes, DNA markers, competent cells, products for RNA analysis and other general reagents for molecular biology. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5 3 polymerase activity (1, 2, 3) and a 5 flap endonuclease activity (4, 5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergentfree and designed to be compatible with existing assay systems.
Homework exercises will be announced in class or listed in the lab manual or on the course web site; 1X NEB buffer 3 100 mM NaCl, 10 mM MgCl2, 1 mM DTT, 50 mM TrisHCl, Taq DNA polymerase is a heat stable enzyme that uses the oligos as primers for DNA synthesis.
View Document Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamerbased inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal cycling conditions, allowing reactions to " OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR DNA polymerases for use with routine and difficult PCR experiments.
The 3 5 exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal Vent DNA Polymerase is a highfidelity thermophilic DNA polymerase.
The fidelity of Vent DNA Polymerase is 515fold higher than that observed for Taq DNA Polymerase (1, 2). This high fidelity derives in part from an integral 3'5' proofreading exonuclease activity in Vent DNA Polymerase (1, 3). Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5 3 polymerase activity (1, 2, 3) and a 5 flap endonuclease activity (4, 5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergentfree and designed to be compatible with existing assay systems. addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3end of PCR products.
The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCRqualified buffer solutions, deoxynucleotides and a broadrange, premixed, readytoload DNA marker to perform 200 Taq polymerase neb manual woodworkers reactions. Taq DNA Polymerase is a highquality recombinant enzyme that is suitable for general and specialized PCR applications (see figures" Tolerance of different primer T m Values" and" Specific amplification of long PCR products" ).